Tm calculation and software

[quin chou]

For oligomers 20-70, best way to calculate Tm is to use nearest-neighbor 
model and actual thermodynamic data. I have developed a software while 
employed at Cetus, which calculate both Tm and predicted anneal 
temperature for PCR (which is the temperature at which 99% template is 
annealed with primers).  I am thinking about puting it into my PCR WWW 
site if there is enough interest.  Now I sell this software at $100, also 
you can get a free copy if you use my service over $1000.

quinnn