Library woes...

[Barry Causier]

I recently obtained a cDNA library in pACT. The library was supplied as a 
bacterial stock in 7% DMSO and was shipped on dry ice. However, by the time I 
received the library the ice had evaporated and the library had thawed. I 
immediately placed the library at -70 having first titred it. When I came to 
use the library the titre had dropped an order of magnitude. How am I supposed 
to store such libraries without losing clones? Clearly I need to know how to 
store it before I go grovelling for another aliquot of the library.

Any thoughts would be gratefully received,

Barry.

+------------------------------------------+
| Hedley Carr, University of Leeds, U.K.   |
| Dept. Biochemistry & Molecular Biology   |
|       BMBTHC at BIOVAX.LEEDS.AC.UK          |
+------------------------------------------+
A little paranoia goes a long, long way....