Sma I religation problem

Amadou Ba PHYASB
Wed Jul 19 13:59:20 EST 1995


Adding Klenow and dNTP would make the overhang in the other end 
blunt and the insertion may occur in both direction ?

We're doing EcorI and SmaI to insert a fragment digested with
EcorI and StuI (blunt end).... Each enzyme linearize the corresponding
vector when used alone. But like in the original article we get to 
many colonies from the control ligation (no insert).
If we understand correctly, adding Klenow and dNTP will make 
every thing blunt ended.


Amadou Ba and Alan Neely

Texas Tech University HSC
Lubbock Texas
phyasb at ttuhsc.edu





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