Help needed in screening libraries.

Richard Hastings mbxrh at unicorn.ccc.nottingham.ac.uk
Thu Jul 20 06:05:18 EST 1995


Does anyone have any idea how many plaques I need to screen to have a
good chance of finding a full length gene that I'm after?  It's a moth
cDNA library cloned in lambda phage.  Also how many plaques is it best
to use on a single plate of 150mm diameter?
Thanks in advance for any help you can give me.

Richard Hastings (mbxrh at unicorn.nott.ac.uk)
Dept. of Biochemistry,
Queens Medical Centre,
University of Nottingham,
Nottingham NG7 2UH.



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