cosmid stability

brian kent bkent at scripps.edu
Thu Jul 20 16:29:53 EST 1995


In article <wjia.1156500368A at news.ucs.ubc.ca>, wjia at unixg.ubc.ca (William
Jia) wrote:

> We are trying to use cosmid to accomodate a large fraction of viral DNA but
> found it is very unstable. I would appreciate if anybody can tell me any
> other system beside cosmid that can accomodate a large DNA fragment but also
> stable.
> Thanks.


Most of the deletions occur after the bacterial culture has reached
plateau stage of growth.  Try the cosmids again but try growing the
culture for only 6-8 hours before prepping.  Do not store the culture
before plasmid prepping as cutures sitting at 4 C will delete.

Good Luck.



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