exposing tritium gels
Richard_Heath
heath at mbcf.stjude.org
Wed Jul 19 13:38:14 EST 1995
In article <f.a.cassidy-1807950943510001 at bcs93.bham.ac.uk>, f.a.cassidy at uk.ac.birmingham (Fiona Cassidy) writes:
> I am running SDS PAGE and IEF gels of proteins which I have labelled with
> tritium, and would like to expose wet gels. I am wondering if wrapping
> these gels in cling film/saran wrap/ cellophane or something similar will
> cause any problems with the signal detection? I'd be grateful to hear if
> anyone has done this in the past
> thanks
I think your main problem is going be the *wet* gel. As far as I am aware,
tritium does not have the energy to escape from wet gels, so adding a layer of
cling film won't make that much of a difference! I think you are going to have
to dry them first.
Richard
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