exposing tritium gels

Richard_Heath heath at mbcf.stjude.org
Wed Jul 19 13:38:14 EST 1995


In article <f.a.cassidy-1807950943510001 at bcs93.bham.ac.uk>, f.a.cassidy at uk.ac.birmingham (Fiona Cassidy) writes:
> I am running SDS PAGE and IEF gels of proteins which I have labelled with
> tritium, and would like to expose wet gels. I am wondering if wrapping
> these gels in cling film/saran wrap/ cellophane or something similar will
> cause any problems with the signal detection?  I'd be grateful to hear if
> anyone has done this in the past
> thanks

I think your main problem is going be the *wet* gel.  As far as I am aware,
tritium does not have the energy to escape from wet gels, so adding a layer of 
cling film won't make that much of a difference!  I think you are going to have
to dry them first.

Richard



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