sequencing

To: Nikolai troianovsk_s at MSDISK.WUSTL.EDU
Thu Jul 20 12:48:02 EST 1995


>Date:   18-JUL-1995 12:38
>From: sabine.hanelt at medizin.uni-ulm.de (Sabine Hanelt)
>Description: sequencing
>
>Hey all there,
>we are sequencing with 35S and that for a long time allready without any 
>problems. The gel is dried on a glas plate and is then exposed to an X-ray 
>film. Since 4 weeks (or so) the film always sticks to the gel, even if the gel 
>was really dry before. We think that one problem might be that there is still 
>some urea in the gel, so that water is taken up again. But we did not change 
>any handling with acetic acid to remove the urea. Does anyone has an idea how 
>we can solve this problem?, or does anyone knows this problem by him/her-self?
>Or has it something to do with temperature, summer, or......?
>Thanks in advance for your help
>Sabine


Hi, there

We used to cover the gel by SaranWrap before drying, and never ever removed
it from gel. 35S goes easyly through SaranWrap, and film never sticks.

Hope this some of help.
Nikolai
http://128.252.119.253





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