DMSO in CaCl2 Competent Cells
howe at ORCHID.UCSC.EDU
Thu Jul 20 12:43:17 EST 1995
DMSO increases the competence of both prokaryotic and eukaryotic cells
(see Chung, CT and RH Miller  Nucleic Acids Research V16: 3580; and
Lopata, MA et al [ 1984] Nucleic Acids Research V12: 5707), although it is
also toxic to cells.
As for the mechanisms of induced DNA translocation into cells using
cation-induced, CaCl-induced, or electroporation, check out the review by
AG Sabelnikov (Prog. Biophys. molec. Biol.,  V62:119-152) entitled,
NUCLEIC ACID TRANSFER THROUGH CELL MEMBRANES: TOWARDS THE UNDERLYING
"The onions expressed here are my own"
On 19 Jul 1995, Dima Klenchin wrote:
> In article
> <gddavis-1907951835120001 at ppp206.modems.uoknor.edu>,
> gddavis at essex.ecn.uoknor.edu (Greg Davis) wrote:
> >Can anyone tell me what the function of DMSO is in the
> preservation of
> >frozen competent CaCl2 competent cells?
> My understanding is that it's not for preservation but for
> the competence. As nobody knows what how E.coli
> uptakes DNA, I guess it's a mistery what DMSO does here.
> (For that matter: what CaCl2 does and how???)
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