How to control the PCR where genomic DNA as a template

giorgio spagnol at galactica.it
Thu Jul 20 04:16:10 EST 1995


In article <gina_briscoe-1307950125220001 at harmonica.cellbio.duke.edu> G.
Briscoe, gina_briscoe at cellbio.duke.edu writes:
> I want to fish out a new gene from bacteria  by PCR where genomic DNA
was
> used as a template and degenerated primers were applyed. I dont know if
I
> need predigest geomic DNA before PCR. If so or not, how to control the
> raction. Thanks.

You do not need to. For Human Genomic DNA, far more complex, I have good
results with 10% DMSO and by denaturing at 95! for 5'.

Best wishes

Giorgio



More information about the Methods mailing list