Artefacts on SDS PA gels

Scott Cowan soc at aber.ac.uk
Thu Jul 20 16:59:17 EST 1995


In article <3uk9cn$a0g at dingo.cc.uq.oz.au> Ian Ross <I.Ross at mailbox.uq.oz.au> writes:
>From: Ian Ross <I.Ross at mailbox.uq.oz.au>
>Subject: Re: Artefacts on SDS PA gels
>Date: 20 Jul 1995 00:52:39 GMT

>HI - I didn't see the original query but I can verify that these bands 
>are almost always due to keratin - usually from human fingers but 
>sometimes from the tissue source.  If from humans, the only solution is 
>scrupulous cleanliness.  Usually this becomes a problem with silver 
>stained gels which of course are so much more sensitive than coomassie 
>gels.

>Regards

>Ian Ross
>Centre for Molecular and Cellular Biology
>University of Queensland
>Australia 4072

You could also try filtering the mercapto-ethanol through 0.2 micron filters, 
this seems to remove a lot of the artifactual bands in silver staining.

Scott




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