GST-Fusion proteins

[Hiranya Roychowdhury]

On Wed, 19 Jul 1995, Frances Mcfarland wrote:

> I have a GST fusion protein that I want to run on a preparative gel.
> Two questions:
> 
> 1) Do I prepare the cells in the same way as for a miniprotein gel, only
> scaled up?

YES

> 2) once I've run the gel, how do I get the protein out of the gel?

By elution. There are a number of ways. Any book on electrophorsis or 
protein chemistry will tell you about the procedures. If you need further 
help you can email your questions.

> 
> I would appreciate any suggestions you may have.
> 
> Thank you
> Frances McFarland
> 
> 


			>>>>>>>>>>>>>>>>>>>>>>>>>>>
			  Hiranya S. Roychowdhury
   			  Plant Genetic Engineering Lab.
			  Box 3GL, NM State Univ.
			  Las Cruces, NM 88003
			  Phone: (505) 646-5785
			  hroychow at nmsu.edu
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