Lysing yeast spheroplasts

Anne Spang spang at vms.biochem.mpg.de
Thu Jul 20 08:35:33 EST 1995


Hi Julie !

I wonder, why you would like to replace DTT by ß-SH! DTT has 2 reactive
sites, while ß-SH contains just one. So you have to double your final
concentration!

Hope this helps!

      Anne



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