Calculating Tm - Help Needed

[Laurentiu COCEA]

HARDIES at THORIN.UTHSCSA.EDU wrote:

> 
> > There does not appear to be a simple solution to the Tm of
> > oligos question. The best method seems to be the nearest neighbor method
> > which is not easily done with a calculator. The software Oligo uses it but
> > the last time I checked, it was $640. Anyone knows of a less expensive
> > software that uses the nearest neighbor method? Primer is free but I'm not
> > sure about the method used.
> 
For the nearest-neigbor method try http://alces.med.umn.edu/rawtm.html.
It seems to work fine.