TA cloning problems!!!!
fisher at radonc.unc.edu
Fri Jul 21 11:29:37 EST 1995
Has anyone had problems TA cloning PCR products into a SmaI digested plasmid?
Briefly, I digested Bluescript II SK + with SmaI, T-tailed it with Taq
polymerase, and ligated my genecleaned pcr product in with T4 DNA ligase.
Alas, no colonies. I have been told that this is a good protocol, and I was
wondering if the problem was due to the digest, since I have heard iffy things
about ligations into SmaI sites.
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