Help needed with FISH
gareth at bioch.ox.ac.uk
Fri Jul 21 05:56:04 EST 1995
I am trying to perform FISH using extended chromatin fibres, using a
selection of cosmid probes labelled with digoxygenin and biotin.
However, while I can get the probes working fine on metaphase
chromosomes, I seem to get either no signal, or just non-specific
binding on the extended chromatin.
If anyone can suggest a means to overcome this, or supply me with a
protocol, I would be most grateful to be emailed the information.
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