5'-RACE

Soren K. Rasmussen soren.rasmussen at risrms1.risoe.dk
Fri Jul 21 03:16:06 EST 1995


We have used the GIBCO 5'-RACE kit and got what we were looking for. The two 
gene specific primers we had to design from a GC-rich region, which may 
explain that less than 5 % of the clones were the right stuff. The poly-A RNA 
were actually 3 years old kept at -80 C

soren k. rasmussen

soren.rasmussen at risoe.dk




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