GC rich riboprobes

Alan Hair pdzah at pdn1.gene.nottingham.ac.uk
Fri Jul 21 11:41:45 EST 1995

Does anyone know of anyway of disrupting secondary structures (due to high
GC content) in riboprobes. I've tried 60¡C in 80% formamide hybridisation
with and without target RNA and only get bands due to self protection.
Any ideas would be appreciated

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