Billy B. Long
blong at lonestar.jpl.utsa.edu
Fri Jul 21 17:04:59 EST 1995
I've never dried a gel to glass, but we dry ours on Whatman paper with
saran wrap over it, remove the saran wrap pafter drying, and sprinkle
baby powder on the gel/paper to keep it from sticking to the film. Never
On 20 Jul 1995 troianovsk_s at MSDISK.WUSTL.EDU wrote:
> >Date: 18-JUL-1995 12:38
> >From: sabine.hanelt at medizin.uni-ulm.de (Sabine Hanelt)
> >Description: sequencing
> >Hey all there,
> >we are sequencing with 35S and that for a long time allready without any
> >problems. The gel is dried on a glas plate and is then exposed to an X-ray
> >film. Since 4 weeks (or so) the film always sticks to the gel, even if the gel
> >was really dry before. We think that one problem might be that there is still
> >some urea in the gel, so that water is taken up again. But we did not change
> >any handling with acetic acid to remove the urea. Does anyone has an idea how
> >we can solve this problem?, or does anyone knows this problem by him/her-self?
> >Or has it something to do with temperature, summer, or......?
> >Thanks in advance for your help
> Hi, there
> We used to cover the gel by SaranWrap before drying, and never ever removed
> it from gel. 35S goes easyly through SaranWrap, and film never sticks.
> Hope this some of help.
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