PCR in ten minutes!
jpcd0 at mole.bio.cam.ac.uk
Sat Jul 22 06:35:55 EST 1995
has anybody out there tried the 'Rapidcycler' from Idaho technology?
We recently got a flier from BioGene Ltd advertising this machine. It
claims to have a ramp time of 10C per second, using 'high velocity
turbulent air'. They include a picture of a gel with a 536bp band
amplified using 30 cycles of 94C 0 sec, 60C 0 sec, 72C 5 sec. Total
reaction time 9 min 25 sec.
This would obviously be seriously handy for cycle sequencing/ optimizing
PCR's and so on.
Has anyone tried this contraption? I haven't called them yet but the flier
does not include cost or size info.
John Dixon Lab 44 (1223) 334131
Wellcome/CRC Institute Fax 44 (1223) 334134
Department of Genetics
United Kingdom e-m: jpcd0 at mole.bio.cam.ac.uk
More information about the Methods