PCR Problem

walter wlech at medicine.medsch.ucla.edu
Sat Jul 22 18:47:39 EST 1995


I always do a "hot start"; add a mix of Taq/10x buffer AFTER 4 min of 
your 5 min denaturing step. Be VERY careful adding reagents while tubes 
are in the machine. Whether this will help your particular situation is 
uncertain, but it can't hurt...





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