Northern blotting

Michael J. Tuvin mtuvin at bcm.tmc.edu
Sat Jul 22 16:07:40 EST 1995


In article <3ue8nn$f8l at azure.acsu.buffalo.edu> v115t2vq at ubvms.cc.buffalo.edu writes:
>A straight and simple question:
>Is there any probe better than rRNA/actins in Northern blot for internal 
>control?
>Thank you!
>Larry Lu
>Dept. of Biochem. Pharmac.
>SUNY at Buffalo
>V115T2VQ at ubvms.cc.buffalo.edu

Sure. cRNA/GAPDH or cDNA/GAPDH. Glyceraldehyde 3-Phosphate Dehydrogenase is
less abundant and more uniform then actin.
We by our probe from Clontech and very happy with it. 
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Michael J. Tuvim 
Research Assistant Professor, Dept.of Medicine, Baylor College of Medicine.
Tel: 713/794-7794(5), FAX: 713/794-7853, e-mail: mtuvin at bcm.tmc.edu
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