ttha at uhura.cc.rochester.edu
Sun Jul 23 22:04:46 EST 1995
In <steinhof.6.00242593@???.Uni-Marburg.DE> steinhof@???.Uni-Marburg.DE writes:
>Could anyone give me a simple procedure for the preparation of histones from
>I have tried it with a hydroxylapatite column, but this did not work very well.
>You can post your answer in this newsgroup a send an e-mail to:
>steinhof at mailer.uni-marburg.de
>Thanks a lot for your help,
How pure do you want the histones? For most cells, simple acid extraction
works fine. Take whole nuclei and resuspend them in 0.4 N H2SO4 in
the cold with shaking or rocking for an hour. Histones are some of
the few acid extractable nuclear proteins.
Tom Thatcher | You can give a PC to a Homo habilis,
University of Rochester Cancer Center | and he'll use it, but he'll use it
ttha at uhura.cc.rochester.edu | to crack nuts.
More information about the Methods