DMSO in CaCl2 Competent Cells

Tim Barnett T.Barnett at
Mon Jul 24 14:21:20 EST 1995

As far as I know, the Ca2+ ions coat the cells providing a net +ve charge
which facilitates the binding of DNA.  Also this coating makes the cell
wall/membrane less stable allowing the DNA to be taken into the cells
following heat shock.


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