Optimal conditions for PCR of small inserts

Oudam Em em at aclcb.purdue.edu
Sun Jul 23 09:22:12 EST 1995

I'm trying to amplify a 50nt insert from soybean cDNA using degenerate
primers.  Has anyone had a similar experiment that they might be able
to offer me some suggestion about finding the right conditions for this
type of amplification?  (em at aclcb.purdue.edu) 

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