[Q] How to purify small PCR frg. ?

Giorgio Spagnol spagnol at galactica.it
Sun Jul 23 02:17:08 EST 1995


In article <950719.111158.19418 at macpost.lidac.liu.se> Sailesh
Surapureddi, SaiSu at MCB.LiU.SE writes:
> >Hi netters,
> >
> >recently a coworker of mine ran into a problem:
> >She created a library with PCR using randomized primers,
> >then she digested the fragment and want to ligate it into a vector.
> >The complete PCR fragment is 96bp, after digestion she gets
> >two fragments: 29 and 67bp. She separated them by gel
> >electrophoresis on a 2.5% AG.
> >The problem is now: How to purify the bigger fragment from the
> >gel slice with a really *high* yield?
> >She tried freeze-and-squeeze and Qiagen-gel-ex but got
> >only very low yields.
> >Any suggestions?

I would try electroeluting them from polyacrilamide gels. However, I do
not know what does it means for you *high* yeld.



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