exposing tritium gels

Bart Sefton Bart_Sefton at QM.Salk.Edu
Mon Jul 24 15:43:05 EST 1995


In article <Pine.SUN.3.91.950721083342.22235A-100000 at verdi>,
hroychow at NMSU.EDU (Hiranya Roychowdhury) wrote:

> With fluors, the secondary emissions do a lot to 'enhance' the signals. 
> However, I am not sure if a an intensifying screen helps, since I don't 
> see how the emissions could penetrate the film with strong enough 
> velocity (if any) to excite the emulsion on the intensifier. I have 
> traditionally left out screens while exposing materials labeled with 
> 35S, 14C and 3H. Was I wrong in assuming that the screens would be redundant.

You were right in assuming that screens don't help.  They augment only 32P
and 125I.  They are too insensitive to enhance weak betas such as those
emitted by 3H, 14C, or 35S.

Bart



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