Sequencing from cDNA
Ann M. Yezerski
ayezersk at moose.uvm.edu
Mon Jul 24 13:16:32 EST 1995
I need some suggestions on methods for sequencing directly from cDNA
clones. I realize that Cycle Sequencing with P 32 is very popular.
However, I am searching for a reliable method using either S 35 or some
non-radioactive method. Has anyone out there used the Sequenase Kit for
such a situation? I do not necessarily need to sequence the whole
contained gene; just the first 50-100 bp for primer possibilities.
Any suggestions and advice send to: ayezersk at moose.uvm.edu
Thank you very much in advance.
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