Best way to get DNA from gel bands?

Allen Gathman C741SCB at SEMOVM.SEMO.EDU
Mon Jul 24 15:04:32 EST 1995

We have been extracting DNA from bands in agarose gels by electroeluting
into a trough in the gel, filled with 15% PEG.  While this works
reasonably well for many people in the lab, it requires some manual
dexterity to cut the troughs, and recovery is not always very good from
the PEG solution.

I know there are numerous kits, spin columns, etc. for this purpose.
What is your experience with these?  What's the best (and reasonably
cheap) method for getting DNA out of a band in a gel?

|    Allen Gathman                |
|    Biology Department           |
|    Southeast Missouri St. U.    |
|    Cape Girardeau, MO 63701     |

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