inclusion bodies

Volker Seibert ifag at po.uni-stuttgart.de
Mon Jul 24 08:57:21 EST 1995


Hi everyone!
I want to express a protein from a gram (+) strain (Rhodococcus erythropolis 
1CP) in E. coli BL 21 (DE3)pLysS. I am using pET11a and pRSET6a vectors. 
Unfortunately, I get most of the protein in inclusion bodies. I have tried 
several conditions including different temperatures (25, 30 and 37øC), 
additives (betaine, sorbitol) and media (M9 and dYT). Renaturation from 
inclusion bodies was not successful. Can anyone help me?
Thanks in advance.
Volker



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