how to make skim milk soluble?

[Bob Belas]

In article <3v007m$b3f at worak.kaist.ac.kr> hbkim at biochem.kaist.ac.kr (Hanbok Kim) writes:
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>From: hbkim at biochem.kaist.ac.kr (Hanbok Kim)
>Newsgroups: bionet.molbio.methds-reagnts
>Subject: how to make skim milk soluble?
>Date: 24 Jul 1995 11:29:58 GMT
>Organization: Biochemistry Lab, KAIST
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>Solid media containing 1% skim milk is used for screening
>the bacterial strain which produces protease. The problem
>is that the skim milk is not solube and precipitated under
>the media. How can it soluble?


Grocery brands of dry skim milk (like Carnation) are soluble at 1%, however 
you have to autoclave the skim milk separately from the rest of the medium 
then add the two solutions together after autoclaving.  If you autoclave 
everything together you get a lot of precipitate.


Bob Belas, Marine Microbial Genetics
      Belas at mbisgi.umd.edu
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