how to make skim milk soluble?

Bob Belas belas at mbisgi.umd.edu
Mon Jul 24 10:56:30 EST 1995


In article <3v007m$b3f at worak.kaist.ac.kr> hbkim at biochem.kaist.ac.kr (Hanbok Kim) writes:
>Path: hecate.umd.edu!haven.umd.edu!purdue!gatech!news.uoregon.edu!news.dacom.co.kr!usenet.seri.re.kr!news.kreonet.re.kr!worak.kaist.ac.kr!biochem.kaist.ac.kr!hbkim
>From: hbkim at biochem.kaist.ac.kr (Hanbok Kim)
>Newsgroups: bionet.molbio.methds-reagnts
>Subject: how to make skim milk soluble?
>Date: 24 Jul 1995 11:29:58 GMT
>Organization: Biochemistry Lab, KAIST
>Lines: 4
>Message-ID: <3v007m$b3f at worak.kaist.ac.kr>
>NNTP-Posting-Host: biochem.kaist.ac.kr
>X-Newsreader: TIN [version 1.2 PL2]


>Solid media containing 1% skim milk is used for screening
>the bacterial strain which produces protease. The problem
>is that the skim milk is not solube and precipitated under
>the media. How can it soluble?


Grocery brands of dry skim milk (like Carnation) are soluble at 1%, however 
you have to autoclave the skim milk separately from the rest of the medium 
then add the two solutions together after autoclaving.  If you autoclave 
everything together you get a lot of precipitate.


Bob Belas, Marine Microbial Genetics
      Belas at mbisgi.umd.edu
..........................................................
"If A is a success in life, then A equals x plus y plus z.
Work is x; y is play; and z is keeping your mouth shut."
Albert Einstein in the Observer January 15, 1950
..........................................................





More information about the Methods mailing list