in vitro tranlation of retroviral gene

Mary P. Remington mremingt at umabnet.ab.umd.edu
Mon Jul 24 07:01:37 EST 1995


We are interested in the invitro tranlation of a retroviral gag/pol 
gene.  We are currently trying the Retic Lysate IVT by Ambion.  I am 
wondering if anyone has experience doing in vitro translation.  Did you 
use Retic Lysate, Wheat Germ, or Untreated Retic Lysate?  Any opinions, 
suggestions etc. appreciated.  Regards, Mary




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