Tunicamycin & COS7 cells

[Nigel Kenward]

Hiya,
Has anyone use tunicamycin with COS7 cells ? Ideally I want to stop the 
production of GPI anchors while not having any effect on cell viability.
How much Tunicamycin should I use ?
Thanx,
Nige

Nigel Kenward              TEL:+44 (0)115 9249924 ex. 44789
Dept. of Biochemistry      FAX:+44 (0)115 9422225
Queens Medical Centre      Email:mbxnjk at vax.nott.ac.uk
Clifton Boulevard          l
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U.K.                   "Back off man, I'm a scientist!"  -- Bill Murray.