Rf: Sequencing from cDNA
logand at msdos.ensam.inra.fr
Tue Jul 25 04:04:54 EST 1995
Ann Yezerski wrote:
>I need some suggestions on methods for sequencing directly from cDNA
>clones. I realize that Cycle Sequencing with P 32 is very popular.
>However, I am searching for a reliable method using either S 35 or some
>non-radioactive method. Has anyone out there used the Sequenase Kit for
>such a situation? I do not necessarily need to sequence the whole
>contained gene; just the first 50-100 bp for primer possibilities.
>Any suggestions and advice send to: ayezersk at moose.uvm.edu
>Thank you very much in advance.
Why not use 33P - can use in PCR reactions and gives good results?
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