Rf: Sequencing from cDNA

[LOGAND]

Ann Yezerski wrote:

>I need some suggestions on methods for sequencing directly from cDNA
>clones.  I realize that Cycle Sequencing with P 32 is very popular.
>However, I am searching for a reliable method using either S 35 or some
>non-radioactive method.  Has anyone out there used the Sequenase Kit for
>such a situation?  I do not necessarily need to sequence the whole
>contained gene; just the first 50-100 bp for primer possibilities.

>Any suggestions and advice send to: ayezersk at moose.uvm.edu
>Thank you very much in advance.

Why not use 33P - can use in PCR reactions and gives good results?

DCL
Montpellier