LPS protein

Len Moaven profpuk at werple.mira.net.au.
Tue Jul 25 00:17:49 EST 1995

Hi everyone, we need help and advice. We have prepared a synthetic
peptide antibody to an outer membrane protein. On Westerns in an
unheated sample this antibody picks up a 36Kd LPS protein (wavy profile
on blot). However, on heating the antigen we detect a smaller product
with a sharp profile. Can any LPS be heat sensitive? Is there any other
modification which will produce the typical LPS profile in gels?

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