Auxotrophic Mutants

John Fox J.R.Fox at shu.ac.uk
Tue Jul 25 07:10:54 EST 1995


I,m trying to produce auxotrophic mutants of A.fumigatus and A.niger.
The method that I intend to use is firstly to harvest spores from each organism
and secondly to expose these spores to UV radiation in 30s bursts upto 10mins in
order to produce a kill curve.  Can anyone offer me any advice on this technique and
comment on any problems that I may encounter and the ways to overcome them?

Thanks in advance,
John



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