Best way to get DNA from gel bands?
nwalker at welchlink.welch.jhu.edu
Tue Jul 25 21:05:24 EST 1995
Use the squeeze method (Biotechniques 1994)...
Place your gel frag in a 3 ml syringe with 100 ul TE
Squeeze it through a 0.22 um syringe filter unit
Rinse thriough with 100ul TE
(At this point you can collect all the filtrate and resqueeze it back
through the filter a few times to increase yield without increasing
Precipitate the DNA and away you go...
We use it routinely now and is essentially the same as the Amicon spin
units(which are probably about the same price when you add it all
up?!!). Yield is about 50%
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