Sequencing from cDNA

Nigel Walker nwalker at welchlink.welch.jhu.edu
Tue Jul 25 20:53:16 EST 1995


Are these clones in plasmids, if so then just do ds sequencing with 
sequenase as normal.

As for cycle sequencing I used S35 not P32 and it worked fine, both on 
plasmid clones and PCR products.(Less stress with the radiation as well 
:-)) FYI it was the Promega cycle sequencing kit that I used. Its not as 
sensitive as P32 but a 3 day exposure is fine under most conditions

Nigel Walker





More information about the Methods mailing list