nuclear localization/ mammalian vector

[Peter Horn]

Hello Netters,

	We have a need for a mammalian expression vector, preferably SV40 or CMV
driven, that allows fusion of a protein of interest to a known nuclear
localization sequence (say of SV40 TAg for example).  Has anyone out there
constructed such a vector for a positive control of nuclear localization? 
Or is one available commercially?  We have not run across one.  Thanks for
any help you can provide.

Peter Horn

hornpete at student.msu.edu

Michigan State University, Dept. of Biochemistry