luciferase assay

Anton Scott Goustin asg at cmb.biosci.wayne.edu
Wed Jul 26 15:04:41 EST 1995


af930 at FreeNet.Carleton.CA (Changcun Jia) wrote:
>
>Does anybody know if there's a way to study spatial expression of luciferase?
>
In a whole-mount or tissue section?  That would be difficult to get the reagents (ATP, Acetyl CoA, luciferin) to the enzyme, unless you use the luciferase+ protein used in Promega's pGL3 series of vectors, which encodes a cytosolic luciferase.  Have you check with Promega?




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