Photographing gels

[CK NG]

> 
> I assume that you are using EtBr to stain the DNA.
> If so, you are using the wrong filter.  I don't
> know the filter #, but you need a red filter for
> EtBr stained gels.  This would explain why you
> don't see the bands as well on the photograph.
> Actually, in my experience, with the correct filter,
> you see the bands better on the photograph than on
> the transilluminator.  You can purchase the right
> filter from suppliers (such as Fotodyne), or, if
> you can find out the Kodak equivalent, from a local
> camera store.  Good luck.
> 
> 
> Gordon Betts (betts at orion.etsu.edu) wrote:
> : I am having difficulty photographing my agarose gels.  The photos
> : don't look good and you can't see the DNA nearly as well as you
> : can on the transilluminator.  I am using an Ultra-Lum(R) transillum.
> :  and photographing with a Polaroid (R) DS34 camera, yellow #8 filter,
> : 1/4 sec, f22 with a 20 second developing time.
> 
> : Any tips on getting decent photos, preferably publication quality?
> 
> : Thanks,
> : Gordon Betts
> : betts at orion.etsu.edu
> : East Texas State University

I believe the filter number is 23A. One can buy that from TIFFEN 
or some other companies. Using a yellow filter # 15 did not help.

Hope this is of use.

CK Ng