Sequencing from cDNA
Nameer B. Kirma
bionbkx at gsusgi2.gsu.edu
Wed Jul 26 16:50:58 EST 1995
On 26 Jul 1995, Nigel Walker wrote:
> Are these clones in plasmids, if so then just do ds sequencing with
> sequenase as normal.
> As for cycle sequencing I used S35 not P32 and it worked fine, both on
> plasmid clones and PCR products.(Less stress with the radiation as well
> :-)) FYI it was the Promega cycle sequencing kit that I used. Its not as
> sensitive as P32 but a 3 day exposure is fine under most conditions
> Nigel Walker
We use P33 for the Promega PCR sequencing kit. It is less
intense than P32 and labels more efficiently than S35--The
best of 2 worlds. An overnight exposure is usually enough.
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