klenchin at macc.wisc.edu
Thu Jul 27 09:29:23 EST 1995
>I have been having probles drying some SDS-PAGE gels (10%,
1.5mm). The gels
>crack when I use a gel dryer
purchased a gel drying kit (promega)
>(leaving the gels in a frame at room temp) and I still get
cracked gels. What
>am I doing wrong?
Maybe, something's wrong with preservarion buffer. Try
30% MeOH, 3% glycerol, at least 1 hr for 1.5 mm gel ->
cellophane drying. If this does not work, your iodine
makes the gel brittle.
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