gel drying

Dima Klenchin klenchin at macc.wisc.edu
Thu Jul 27 09:29:23 EST 1995


>I have been having probles drying some SDS-PAGE gels (10%, 
1.5mm).  The gels
>crack when I use a gel dryer 
>...
purchased a gel drying kit (promega)
>(leaving the gels in a frame at room temp) and I still get 
cracked gels.  What
>am I doing wrong?
>
Maybe, something's wrong with preservarion buffer. Try 
this:

30% MeOH, 3% glycerol, at least 1 hr for 1.5 mm gel -> 
cellophane drying. If this does not work, your iodine
makes the gel brittle. 

- dima



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