Apa1 digestion

brett brett at BORCIM.WUSTL.EDU
Fri Jul 28 12:28:02 EST 1995


>Regarding your problem with Apa1 cutting a PCR product, I was just trying to
>do the same thing and had no luck.  I had the additional problem that my 
>Apa1 cut (CIPed, and gel purified) vector gave me background.  I tried longer
>digestions, more enzymes, all the usual and nothing worked.  I would also be
>interested in any suggestions that are offered!


Avoid this enzyme. Bsp120I is much better.



Brett Lindenbach
    
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             






More information about the Methods mailing list