Apa1 digestion

brett brett at BORCIM.WUSTL.EDU
Fri Jul 28 12:28:02 EST 1995

>Regarding your problem with Apa1 cutting a PCR product, I was just trying to
>do the same thing and had no luck.  I had the additional problem that my 
>Apa1 cut (CIPed, and gel purified) vector gave me background.  I tried longer
>digestions, more enzymes, all the usual and nothing worked.  I would also be
>interested in any suggestions that are offered!

Avoid this enzyme. Bsp120I is much better.

Brett Lindenbach
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             

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