Apa1 digestion

Susan M. Desimone sdesimon at moose.uvm.edu
Fri Jul 28 11:24:26 EST 1995

Regarding your problem with Apa1 cutting a PCR product, I was just trying to
do the same thing and had no luck.  I had the additional problem that my 
Apa1 cut (CIPed, and gel purified) vector gave me background.  I tried longer
digestions, more enzymes, all the usual and nothing worked.  I would also be
interested in any suggestions that are offered!

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