Ligation problem

Steven Sullivan sullivan at gwis2.circ.gwu.edu
Fri Jul 28 09:09:49 EST 1995


I've been told by rival companies that residual NaI left by products like 
GeneClean can inhibit ligations (esp. blunt-end).  

I use Glass Max, though, which also has NaI, and I have no prblem with 
ligation into TA vectors.  NaI solutions can go bad if exposed to light , 
however, so you might check this.  


For blunt end fragments, I add an A to each end with a little bit of Taq
and a short incubation at 72 degrees -- call Invitrogen, they'll send you
a one-page protocol for this. 




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