how to make skim milk soluble?

"Marianne Leverone ", BIO leverone at CHUMA.CAS.USF.EDU
Fri Jul 28 14:28:10 EST 1995

On 24 Jul 1995, Hanbok Kim wrote:

> Solid media containing 1% skim milk is used for screening
> the bacterial strain which produces protease. The problem
> is that the skim milk is not solube and precipitated under
> the media. How can it soluble?
How long are you autoclaving the medium?  Decrease autoclave time to 
a minimum of 10-15 min.  You may be denaturing the casein.  Marianne, 
Biology Dept., Univ. of South Fla., Tampa, FL 

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