how to make skim milk soluble?
"Marianne Leverone ", BIO
leverone at CHUMA.CAS.USF.EDU
Fri Jul 28 14:28:10 EST 1995
On 24 Jul 1995, Hanbok Kim wrote:
> Solid media containing 1% skim milk is used for screening
> the bacterial strain which produces protease. The problem
> is that the skim milk is not solube and precipitated under
> the media. How can it soluble?
How long are you autoclaving the medium? Decrease autoclave time to
a minimum of 10-15 min. You may be denaturing the casein. Marianne,
Biology Dept., Univ. of South Fla., Tampa, FL
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