MAbs in Bacteria
user at darwin.bio.uci.edu
Fri Jul 28 16:45:47 EST 1995
Thanks for your answer!
Louis Hom <lhom at ocf.Berkeley.EDU>
"I have never heard of people expressing Abs in bacteria (though I can't say
I've really checked, either). I have heard of them being expressed in yeast,
though, since yeast are capable of the necessary glycosylation."
Jeffrey Jackson <jeffj at u.washington.edu>
"Though I haven't tried the protocol described in it yet, the reference
you will need is Carter et al, Bio/Technology 10(163-167) 1992.
My lab has the pAK-19 vector described in the paper. If you can get your
hands on that, a good idea is to clone a 1.7 kb RI/HIII fragment
containing the heavy and light chain genes into pUC-119. Then you can
clone in your heavy and light chains by sticky-foot mutagenesis (at least
in theory - that's what I'm trying to do right now without a whole lot of
If intersted check out:
Bradburry & Cattaneo. TINS. Vol 8 No 6 p 243 (1995) and references therein.
Any other suggestions will be appreciated........ata
email mataulfo at darwin.bio.uci.edu
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