RT-PCR and gels

GORDON BETTS betts at ORION.ETSU.EDU
Fri Jul 28 16:36:49 EST 1995


I believe it is possible to run RT then take 3 ul or so and run
on a 1% agarose gel to verify you have a product. Correct?
If not, then how can you check to see if your RT worked before 
wasting reagents on PCR?
If you can run your RT product on a gel to confirm cDNA is present,
then could you suggest a procedure that is reliable? Mine's not.
Gordon Betts, Ph.D.
Biology Dept.
East Texas State University
Commerce, TX 75429-3011
903/886-5369
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