Apa1 digestion

Philippa Whitelaw pwhitela at uoguelph.ca
Fri Jul 28 14:31:53 EST 1995


Apa1 is sensitive to methylation of bases which overlap the restriction 
site. According to the Promega catalogue (pg 49) (not an employee) the 
dcm gene methylates the C5 residue in DNA sequences of the internal 
cytosine residue in sequences 5' CCTGG 3' and 5'CCAGG 3'... so check your 
sequences! 

Philippa 





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