Help in SDS-PAGE

Giorgio Spagnol spagnol at galactica.it
Sat Jul 29 03:16:40 EST 1995


Hi netters,

Seems that my destiny in these days is to have troubles in one
dimensional SDS-PAGE. Lately, instead of migrating as bands, my sample
form two vertical streaks wich seem to migrate from the sides of the
wells down in the gel. I usually make a 6% gel and run it for two hours.
It makes no difference if I load 10 ug or 10 ng.
Any suggestion? I thank you in advance.
Giorgio



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